Biocompatibility evaluation of an antibiotic paste after pulpotomy in dogs

The purpose of this study was to evaluate by histological analysis, the biocompatibility of an antibiotic paste following treatment of pulpotomies in dogs. Four male adult dogs were used and their mandibular and maxilar premolars and molars (PM3; PM4; M1; PM4;M1) of both sides were submitted to pulpotomies. The antibiotic paste (CTZ) was prepared with chloramphenicol, tetracycline, zincoxide and eugenol. After 6, 8, 9 and 10 months post-pulpotomy the dogs were sacrificed. Histological analysis of the teeth revealed an intense inflammatory process in the coronal pulp at 6 months after pulpotomy, with inflammatory cells dispersed throughout the entireapical region in this period. This process became partially reduced at 8 and 9 months after pulpotomy, and totally disappeared by the end of the experiment, at 10 months. In conclusion, the endodontic treatment with this antibiotic paste seemed to be biocompatible under the present experimental conditions. However, further clinical studies are necessary to assure that this paste is safe for use in pulpotomies in children.

Avaliação hematológicas e bioquímicas do sangue de cães submetidas a pulpotomias com cimento de antibiótico / Hematological and biochemical evaluations of blood from dogs submitted to pulpotomies with an antibiotic sealer

Este estudo experimental avaliou os parâmetros hematológicos e bioquímicos no sangue de 7 cães machos e adultos, com peso variando de 11 a 20 kg, submetidos a pulpotomias dos dentes molares e pré-molares, utilizando-se o cimento de antibiótico composto de cloranfenicol, tetraciclina, óxido de zinco e eugenol, como obturador da câmara coronária. Foram selecionados 10 dentes hígidos de cada cão com boas condições gengivais e as pulpotomias foram realizadas sob anestesia geral. Coletou-se o sangue antes e 48 horas, 30, 60 e 90 dias após as pulpotomias, para as avaliações hematológicas e bioquímicas. Os resultados mostraram que, no exame hematológico, o único parâmetro alterado foi o referente à contagem de plaquetas cujos valores já eram reduzidos antes e após o tratamento endodôntico. O exame bioquímico manteve-se dentro dos valores de referência, no entanto, os valores médios referentes às bilirrubinas mostraram-se elevados, ao final do experimento. Concluiu-se que o uso do cimento de antibiótico, em pulpotomias realizadas em cães, parece ser destituído de efeitos tóxicos, seguindo-se a metodologia testada. Todavia, sua utilização em pulpotomias de dentes humanos decíduos, apesar de promissora, carece de maiores estudos clínicos.

The present work evaluated hematological and biochemical parameters in blood from seven male adult dogs (11 to 20 kg) submitted to molar and pre-molar pulpotomies with an antibiotic sealer. This sealer was prepared with chloramphenicol, tetracycline, zinc oxide and eugenol, and used for the obturation of the coronary pulp chamber. The pulpotomies were performed under general anesthesia, and the blood was collected before and 48 hours, 30, 60 and 90 days after that procedure, for hematological and biochemical evaluations. The hematological evaluation showed that the only altered parameter was that related to platelet count whose values were already reduced before and after the pulpar therapy. Similarly, the biochemical parameters were also maintained at normal levels, except for the bilirubin concentrations which were increased at the end of the experiment. The conclusion was that the use of the antibiotic sealer for pulpotomy, in dogs, seems to be devoid of toxicity under the experimental conditions. However, clinical studies are necessary for assuring the safety of the use of this sealer in pulpotomies in children.